mouse anti chicken cd8a fitc  (SouthernBiotech)

Journal: Frontiers in Physiology

Article Title: Effects of Artemisia annua supplementation on the performance and gut health of laying hens challenged with mixed Eimeria species

doi: 10.3389/fphys.2024.1381548

Figure Lengend Snippet: Representative histogram of the gating protocol used on the enriched chicken peripheral blood lymphocytes stained with PE-CD4 and FITC CD-8. The cells were first gated based on the size (FSC-H) and granularity (SSC-H) (A) . The single cell population was then gated from the histogram (A) based on the cell area (SSC-A) and the cell height (SSC-H) (Fig. 1B). Singlet cells identified in (B) were then analyzed for PE-CD4 and FITC-CD8 staining (C) .

Article Snippet: In brief, 100 μL of the isolated cells were incubated with CD4 (mouse anti-chicken CD4-PE; SouthernBiotech, Birmingham, AL) and CD8 (mouse anti-chicken CD8a-FITC; SouthernBiotech, Birmingham, AL) antibodies.

Techniques: Staining

Journal: Frontiers in Physiology

Article Title: Effects of Artemisia annua supplementation on the performance and gut health of laying hens challenged with mixed Eimeria species

doi: 10.3389/fphys.2024.1381548

Figure Lengend Snippet: Effect of the inclusion of phytogenic feed additive A. annua in the diets of laying hens on their peripheral mononuclear blood cell count and CD4 + and CD8 + T cell population 14 days post- Eimeria challenge. At 25 weeks of age, 12,500 E. maxima , 12,500 E. maxima , and 62,500 E. tenella oocysts were inoculated in laying hens grouped into challenged control, 0.5% A. annua , and 1% A. annua . NC: non-challenged control; PF: pair-fed control; CC: challenged control (12,500 E. maxima ; 12,500 E. tenella ; and 62,500 E. acervulina oocysts per mL); 0.5AA: challenged control with the inclusion of 0.5% A. annua in the diet; 1AA: challenged control with the inclusion of 1% A. annua in the diet; dpi: days post-inoculation of Eimeria spp.

Article Snippet: In brief, 100 μL of the isolated cells were incubated with CD4 (mouse anti-chicken CD4-PE; SouthernBiotech, Birmingham, AL) and CD8 (mouse anti-chicken CD8a-FITC; SouthernBiotech, Birmingham, AL) antibodies.

Techniques: Cell Counting, Control

Journal: Frontiers in immunology

Article Title: Development and characterization of a CRISPR/Cas9-mediated RAG1 knockout chicken model lacking mature B and T cells.

doi: 10.3389/fimmu.2022.892476

Figure Lengend Snippet: FIGURE 6 Immune cell populations and quantification of secreted immunoglobulins in RAG1 knockout chickens. Representative flow cytometry analysis of B and T cell subpopulations in the bursa (A), and thymus (B) of 3-week-old chickens. Statistical analysis was performed on Bu-1 and IgM positive cells in the bursa as well as CD4+ and CD8+ single-positive (SP) T cells, and the CD4+CD8+ double-positive (DP) T cell lymphocyte subpopulation in the thymus. Serum IgM, IgY, and IgA levels in 1-week (C) and 3-week (D) -old WT, RAG1+/-, and RAG1-/- chickens, as determined by ELISA. Each dot represents an individual chicken. The significance of differences among groups was assessed by one-way ANOVA; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, no significance.

Article Snippet: Cells (1 × 106) were stained for 40 min on ice using the following antibodies: mouse anti-chicken Bu-1 FITC (8395-02), mouse anti-chicken IgM BIOT (8310-08), mouse anti-chicken CD45 SPRD (8270-13), mouse antichicken CD3 FITC (8200-02), mouse anti-chicken CD4 Alexa Fluor 647 (8201-31), mouse anti-chicken CD8a BIOT (8405- 08), mouse IgG1-FITC (0102-02), mouse IgM-SPRD (0101-13), mouse IgG2b-BIOT (0104-08) and mouse IgG1-Alexa Fluor 647 (0102-31) were purchased from Southern Biotech (Birmingham, AL, USA).

Techniques: Knock-Out, Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Food and Agricultural Immunology

Article Title: The effect of humic substances on gut microbiota and immune response of broilers

doi: 10.1080/09540105.2019.1707780

Figure Lengend Snippet: Figure 3. The effect of 0.8% of natural humic substances prepared from leonardite in feed on the per- centage of CD4+ CD8–, CD8+ CD4– and CD4+ CD8+ lymphocytes in broiler peripheral blood. Means with different superscripts are significantly different **p < .01.

Article Snippet: Tubes were centrifuged for 30 min at 600× g. MNL were recovered by aspirating white layer in plasma-LSM interphase, and the acquired sample was washed twice with PBS by centrifugation for 5 min at 250× g. Selected lymphocyte subpopulations were identified by direct staining with a subsequent combination of conjugated anti-chicken monoclonal antibodies (MoAb): CD4-FITC/ CD8a-R-PE/CD45-APC (Southern Biotech, USA).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Probiotic Lactobacillus johnsonii BS15 Improves Blood Parameters Related to Immunity in Broilers Experimentally Infected with Subclinical Necrotic Enteritis

doi: 10.3389/fmicb.2018.00049

Figure Lengend Snippet: CD3 + (A) , CD3 + CD4 + (B) , CD3 + CD8 + (C) , T-lymphocyte percentages and CD3 + CD4 + /CD3 + CD8 + ratio (D) in the peripheral blood at days 21 and 42 (Experiment 1). Bars with different letters are significantly different on the basis of Duncan’s multiple range tests ( P < 0.05). Data are presented as mean ± standard deviation (six replicates of one chick per cage).

Article Snippet: The cells were, respectively, stained with 10 μL mouse anti-chicken CD4-phyto-erythrin (BD Pharmingen, United States) and mouse anti-chicken CD8a-FITC (BD Pharmingen) for 15–20 min at RT, and then 2 mL PBS added and centrifugal elutriation performed once.

Techniques: Standard Deviation

Journal: Frontiers in Microbiology

Article Title: Probiotic Lactobacillus johnsonii BS15 Improves Blood Parameters Related to Immunity in Broilers Experimentally Infected with Subclinical Necrotic Enteritis

doi: 10.3389/fmicb.2018.00049

Figure Lengend Snippet: CD3 + (A) , CD3 + CD4 + (B) , CD3 + CD8 + (C) , T-lymphocyte percentages and CD3 + CD4 + /CD3 + CD8 + ratio (D) in the peripheral blood (Experiment 2). Bars with different letters are significantly different on the basis of Duncan’s multiple range tests ( P < 0.05). Data are presented as mean ± standard deviation (six replicates of one chick per cage).

Article Snippet: The cells were, respectively, stained with 10 μL mouse anti-chicken CD4-phyto-erythrin (BD Pharmingen, United States) and mouse anti-chicken CD8a-FITC (BD Pharmingen) for 15–20 min at RT, and then 2 mL PBS added and centrifugal elutriation performed once.

Techniques: Standard Deviation